MBP Antibody- SC1D7

(Catalog # AM001)

Specificity: Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).

Antigen used for immunizations: The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109. The purified recombinant MBP protein was then used for mouse immunization.

Storage Buffer: The ammonium sulfate-precipitated ascites fluid is kept in PBS with 1% BSA and then lyophilized.

Recommendations on use:

Immunofluorescence:

Typical working dilution on frozen sections 1:200. 60 minutes primary antibody incubates at 25ºC (as shown in Fig. 1) (will soon be available).

Western blotting:

Recommended: working dilution on Western bloting 1:500. 60 minutes primary antibody incubates at 25ºC  (as shown in Fig. 2)(will soon be available).

Storage and stability: Store unopened antibody at 4ºC or lower. Under these conditions, there is no significant loss in product performance one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4ºC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20ºC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Legal consideration: FOR RESEARCH USE ONLY.

Reference:

Chuang CH, Hsu SC, Hsu CL, Hsu TC, Syu WJ. Construction of a tagging system for subcellular localization of proteins encoded by open reading frames. J Biomed Sci. 2001 Mar-Apr;8(2):170-5.

Hsu SC, Yan BS, Pan JM, Syu WJ. A monoclonal antibody reacts with maltose-binding protein of Escherichia coli and related enteric bacteria. J Immunol Methods. 1997 May 26;204(2):169-74.

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