Alsin Antibody

(Catalog # AP017)

 Specificity: Rabbit polyclonal antibody against human Alsin (Amyotrophic lateral sclerosis protein 2).

Antigen used for immunizations: Prokaryotic recombinant protein containing seven copies of the amino acid residues 1 to 12 (MDSKKRSSTEAE) of the human Alsin [Accession #: NP_065970].

Storage Condition: Whole serum with 0.1% NaN3.

Recommendations on use:

Immunofluorescence:

Not applicable.

Western blotting: Recommended:

Working dilution on Western blotting 1:500~1000. 60 minutes primary antibody incubates at 25ºC. Loaded dot: PE- 1x Alsin 1-12 (MDSKKRSSTEAE) fusion protein. Antigen: G S T-7x Alsin 1-12. Pre: preserum; 500x: rabbit serum 500X; 1000x: rabbit serum 1000X; 2000x: rabbit serum 2000X.

Positive controls: PE*- 1x Alsin 1-12 (MDSKKRSSTEAE) fusion protein.

                                                         *PE: Pseudomonas aeruginosa exotoxin A receptor binding domain.

Storage and stability: Store unopened antibody at 4ºC or lower. Under these conditions, there is no significant loss in product performance up to one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4ºC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20ºC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Legal consideration: FOR RESEARCH USE ONLY.

Reference:
Hosler BA, Sapp PC, Berger R, O'Neill G, Bejaoui K, Hamida MB, Hentati F, Chin W, McKenna-Yasek D, Haines JL, Patterson D, Horvitz HR, Brown RH Jr, Day CB. Refined mapping and characterization of the recessive familial amyotrophic lateral sclerosis locus (ALS2) on chromosome 2q33. Neurogenetics. 1998 Dec;2(1):34-42.

Yang Y, Hentati A, Deng HX, Dabbagh O, Sasaki T, Hirano M, Hung WY, Ouahchi K, Yan J, Azim AC, Cole N, Gascon G, Yagmour A, Ben-Hamida M, Pericak-Vance M, Hentati F, Siddique T. The gene encoding alsin, a protein with three guanine-nucleotide exchange factor domains, is mutated in a form of recessive amyotrophic lateral sclerosis. Nat Genet. 2001 Oct;29(2):160-5.

Hadano S, Hand CK, Osuga H, Yanagisawa Y, Otomo A, Devon RS, Miyamoto N, Showguchi-Miyata J, Okada Y, Singaraja R, Figlewicz DA, Kwiatkowski T, Hosler BA, Sagie T, Skaug J, Nasir J, Brown RH Jr, Scherer SW, Rouleau GA, Hayden MR, Ikeda JE. A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2. Nat Genet. 2001 Oct;29(2):166-73.

Price

Cat# Item Spec Price (USD, FOB)
AP017 Alsin Antibody 100µl 300